General: Heavy rain on take-off, clear soon after. 22:32 climbing through 26 kft O2 and CO dipped but CO2 fairly constant. Saved PNGs. H2O varying from 0 to 20,000 ppm on sawtooth - for an average of 10,000 ppm over 8 hours at 100 sccm = 48 StdL * 10,000 ppm = 48 StdL / 22.4 StdL /mol * 10,000 * 10^-6 * 18 g/mol / 1 g/cc = 0.4 cc (hopefully OK). But at 3000 SLPM, equals 11 cc (!) - at a 0.8" cross-section On previous flight, O2 in MBL went from 6,000 to 14,000 ppm - see any reduction in flow by end of flight? 00:55 Crossing EQ at 14 kft 01:00 on way down, ATC said we could make 43 kft if we hurried - rushed dip into MBL and ascent, then climb out, only to learn that we would get stuck high at 43 if we went, so staying below 28 kft. AO2: Chaotic preflight - got to initialize cal flow step and SP 1250 failed to open valve. Replaced with S/N 7 (S/N 5 was in). Worked hanging off wires, but failed when mounted. Unmounted and found pin 15 was out and loose on connector - worked out and in (so maybe S/N 5 was fine and just connector was bad). Then tune was way off and took ~15 minutes to fix. Lit lamp ~ 10 minutes before take-off. Set CalInt to 5 and WTfreq to 1 to get two 3-point cals on climb-out. Forgot to reset, so got a third 2-point cal and didn't start sampling air until level @ 40 kft ~ 21:25. 21:47 Still level at 40 kft, pilots having trouble getting clearance for lower. 21:49 on descent, to MAN to delay cal 22:15:58 back to AUTO, purging LS HS is off scale for CO2 on AEROS again (despite programmed offset) - reads 283 ppm on AO2 PC. LT reads 365 on AEROS and 413 on AO2 PC. Suspect pressure calibration is off - running at 325 instead of 330 on this flight. Need to go back to 330 and/or recal Li840 with an N2 zero. LT reads 384 on AEROS, 382 on PC, so cal at WT (385) is fine, it's just the span and zero are way off - need to borrow Fred's N2 for a zero. 00:15:30 PaSP holding at 795, but will monitor for ice blockage 00:18-00:27 Ran a cal exactly between 5 kft and 5 kft on dip - should have delayed. 00:44 Cell vs. bypass flow ~ 5 sccm different - need to adjust HA-3 (or go back to 330 torr?) 00:58:10 to MAN and uncheck LS purge to delay cal 01:44 Forgot to go back to AUTO until 01:44 03:29 PaSP still 795. Cryo T still -74 03:40:13 to MAN to prevent cal Possible photosynthesis signal compounded by O2 motion effect on landing MEDUSA: Because of AO2 problems, preflight hectic as well. Overnight leak rate from start-up: Pup 448, Pdn 491, Pbyp 502. Ran an ~ 1.25 hour leak check (18:30 pull down) from 19:44 - 20:05. Saved PNG. No glaring downstream leaks, but Pup climbed sharply starting from position 8 through to 14 (when it equalled Pdown). Not sure what caused this, but could be a pump flapper freeing up. On initial power up. Display showing Position 23. Valve box power off/on did not resolve and handlinks and LEDs show normal 6-way and Li840 status. After AEROS started, and powered down/up again seems to be fixed. Not sure if AEROS has anything to do with it, perhaps it's just a matter of a power down/up after warmup. . . . NEVER SYNC'D MEDUSA CLOCK: 6 SECONDS FAST ALL FLIGHT (some checksheet times may have accidentally been recorded in MEDUSA time) Flow off at 40 kft. Back up on descent at ~ 21:49. Sampled first flask at 21:56:36 (7.5 min of stable flow) 22:49:35 on position 7, went to bypass to do a leak check (flow recovery looks slow) Pup 140, Pdn 399, Pbyp 707 (!) should be ~ 760. Leak check looked good. Back running ~ 22:57. Next sample at 23:17:24 (20 minutes of stable flow) 23:50 On position 13, noticed flow has dropped. Went to bypass and tried jumpering Trap #1 out. Flow comes back up. Back to flask at 22:52:28. Was running on Traps A (#1) and D (#2). Now running just on D (#1). Drying A and will put back inline as #2 after next profile. At 00:30 and again at 01:00, flow dropping (see water calcs above). Flasks 14, 15, and 16 collected with variable flow. Flask 13 had slightly reduced flow. Flow back up for flask 17 (ice chunks in 1/4" part blasting out?) 01:37 flow down to 3000 01:37:50-01:38:05 Tapped on trap to see if ice could be dislodged - if anything, made worse. 01:39:20, flow down to 2200 and falling. 01:40 Swapped trap D which has been warmed and somewhat (gravity) dried, back in for A as solo trap. Flow up to 3150 again. Plugged traps do not seem to have much solid ice on walls or dip tube, but do have ice in inlet 1/4" - possibly dry ice is too high and inlet tube is getting too cold. . . raised trap D up 1/4" to see if this helps. from 02:00 - MEDH2O shows noticably (~100 ppt) wetter. 02:25 dropped trap back down 1/4" to original position - did not seem to have a drying effect - may have gotten exit tubes from trap wet at some point? forgot to close 1-16 until all done sampling, closed 1-32 @ 03:15